ZnAF®-2/ ZnAF®-2 DA

[Zinc ion imaging probe]

495-540 nm：Green

ZnAF-2, that has structure of fluorescein connected with TPEN analog, has high specificity to Zn²⁺.ZnAF-2 has high solubility, so that it is not permeable through the cell membrane. ZnAF-2 DA has an additional acetyl group which make the reagent go inside the cell much easier, and the acetyl group is removed by the intracellular esterase which make the reagent stay inside the cell for long time.

ZnAF is a registrated trademark of Daiichi Pure Chemical. The ownership of the trademark was inherited by SEKISUI Medical and now is owned by Goryo Chemical, Inc.

Available through Merck KGaA (Darmstadt, Germany) as:
SCT 031 BioTracker™ 515 Green Zn2+ Dye
SCT 032 BioTracker™ 515 Green Zn2+ DA Dye

Products

Code No.	Product Name	Size	Merck CAT No.	Merck ( Millipore / Sigma Aldrich ) Product Name
SK2001-01	ZnAF®-2	1 mg	SCT031	BioTracker 515 Green Zn2+ Dye
SK2002-01	ZnAF®-2 DA	1 mg	SCT032	BioTracker 515 Green Zn2+ DA Dye

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Protocol
SK2001-01 SDS
SK2002-01 SDS

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Product Information

ZnAF®-2/ ZnAF®-2 DA
Product Information

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Propertites of ZnAF-2

Product Name	target	cell permeability	reaction	Abs_max (nm)	FL_max (nm)	K_d for Zn²⁺ (nM)	ε	Φ
ZnAF-2	Zn²⁺	no	reversible	492	514	2.7	120,000	0.39
ZnAF-2 DA	Zn²⁺	yes	reversible	492	514	2.7	120,000	0.39

Features

High affinity to Zn²⁺that enables us to detect low concentration of Zn²⁺(dissociation constant: 2.7 nM).
High specificity to Zn²⁺ in the buffer.
Low background fluorescence contributes high sensitivity of Zn²⁺in living sample.
ZnAF-2 DA is permeable through the cell membrane, so that by using the reagent, bioimaging in living cell or tissue is available.
ZnAF-2 DA is deacetylated by esterase in the cell, and it stays inside the cell for long time.