POLARIC-BCS is a solvatochromic dye for staining bacteria. It stains both gram-positive and gram-negative bacteria cell membranes.
For laser excitation, 488 nm or 514 nm is applicable.
POLARIC® Series
POLARIC®-500BCS
[Bacterial membrane detecting probe]
Others
Products
| Code No. | Product Name | Size | Merck CAT No. |
Merck ( Millipore / Sigma Aldrich ) Product Name |
|---|---|---|---|---|
| GC102 | POLARIC®-500BCS | 5 μg × 10 | ||
| GC1021 | POLARIC®-500BCS | 5 μg × 5 |
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Product Information
POLARIC®-500BCS
Product InformationPrint
Properties of POLARIC-500BCS
Product name
target reaction Absmax (nm)* FLmax (nm)* POLARIC-500BCS solvent polarity reversible 480 592 *Because of low water solubility of POLARIC-500BCS, it is a value determined in aqueous medium containing SDS. It changes depending on the solvent polarity.
An example spectra in bacterial cell membrane
For laser excitation, 488 nm or 514 nm is applicable.
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Imaging examples with POLARIC-500BCS
POLARIC®-500BCS
Imaging examples with POLARIC-500BCSPrint
Imaging examples with POLARIC-500BCS
Salmomella typhimurium stained with POLARIC-500BCS were imaged wth structured illumination microscopy (Nikon, N-SIM). Ex: 488 nm, Em: 510-550 nm. Membrane structure of the bactria were clearly observed.
FAQ
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Q Can I selectively stain bacterial membranes with POLARIC-500BCS?A
No. POLARIC-500BCS also stains eukaryotic cells including mammalian culured cells. You can not selectively stain prokaryotes.
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Q In what situation does POLARIC change the color?A
Reference
Y. Kawasaki, T. Endo, A. Fujiwara, K. Kondo, M. Katahira, T. Nittami, M. Sato, M. Takeda (2016)
Int. J. Biol. Macromol. 95: 1280-1288 DOI:10.1016/j.ijbiomac.2016.11.025
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