TokyoGreen®–βGlcU(Na)

[β-glucuronidase activity detection probe]

495-540 nm：Green

TokyoGreen-βGlcU(Na) is cell permeable fluorescent substrate [9- (4-methoxy-2-methylphenyl) -6-oxo-6Hxanthen-3-yl-β-D-glucuronide, sodium salt] for detecting β-glucuronidase. Non-fluorescent TokyoGreen-βGlcU(Na) is hydrolyzed by the β-glucuronidase, and generates bright fluorescent substance, TokyoGreen^®.

The product has been suspended to sell.
The schedule of resumption has not been fixed.

Available through Merck KGaA (Darmstadt, Germany) as:
SCT027 BioTracker™ 510 Green β-Glu (Na) Dye

Products

Code No.	Product Name	Size	Merck CAT No.	Merck ( Millipore / Sigma Aldrich ) Product Name
SK4003-01	TokyoGreen®–βGlcU(Na)	1 mg	SCT027	BioTracker 510 Green β-Glu (Na) Dye

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Product Information

TokyoGreen®–βGlcU(Na)
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Principle of detection

Non-fluorescent TokyoGreen^®–βGlcU(Na) is hydrolyzed by the β-glucuronidase , and generates bright fluorescent TokyoGreen^®. TokyoGreen^® has bright green fluorescence (510 nm) when it is irradiated by the 490 nm excitation light.

Contents

TokyoGreen^®-βGlcU(Na) 1mg (5 mM in DMSO 0.38mL)
C₂₇H₂₃NaO₁₀ MW：530.46

FAQ

Q Can I use for the fixed samples?

A

You may use a similar procedure to X-gal staining for fixed cell samples, because GlycoYELLOW-βGal is also a substrate for β-galactosidase (lacZ reporter)

We confirmed that fluorescence of GlycoYELLOW-βGal observed in live cells does not diminish by the normal process of fixation. Please refer information in the product page for details.

Strong fixation conditions may alter the intracellular localization or may decrease the fluorescence. Please test the fixation condition in your cells and conditions.
Q Give me a selection guide of the β-galactosidase probes.

A

We have three β-galactosidase probes, GlycoGREEN-βGal, GlycoGREEN-βGal, and TokyoGreen-βGal . For cell imaging, we recommend GlycoGREEN-βGal, for plate-reader assays or for enzyme kinetics measurements, we recommend TokyoGreen-βGal as a first choice.

Sensitivity of the probe isGlycoGREEN-βGal ~ TokyoGreen-βGal > GlycoYELLOW-βGal.
For cell imaging, we recommend GlycoGREEN-βGal because its cell retention is relatively better, however, generated fluorophore also leaks from the cells and can be incorporated into other cells, users should carefully check the result when they observe heterogeneous cells.
Q My question is not in this FAQ list.....

A

Please also refer the frequently asked questions on fluorescent probes