EP-HMRG is almost nonfluorescent material at pH 7 or higher. It fluoresces by the reaction with proteases that can digest the peptide sequence of EP (glutamic acid, proline). With this reagent, users can detect the cells and tissues that overexpresses the protease by green fluorescence.
ProteoFluor™ Series
EP-HMRG
[Protease detecting fluorescent probe]
Products
| Code No. | Product Name | Size | Merck CAT No. |
Merck ( Millipore / Sigma Aldrich ) Product Name |
|---|---|---|---|---|
| GC811 | EP-HMRG | 30 nmol x 5 |
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Product Information
EP-HMRG
Product InformationPrint
Properties of EP-HMRG
Product Name
reaction Absmax (nm) FLmax (nm) ε Φ EP-HMRG irreversible 500 524 57,000 0.81 The above data is that of after the reaction with proteases.
Spectra
Spectra measured in PBS (pH 7.4)
Reference
H. Onoyama, M. Kamiya, Y. Kuriki, T. Komatsu, H. Abe, Y. Tsuji, K. Yagi, Y. Yamagata, S. Aikou, M. Nishida, K. Mori, H. Yamashita, M. Fujishiro, S. Nomura, N. Shimizu, M. Fukayama, K. Koike, Y. Urano, Y. Seto (2016)
Sci. Rep. 6: 26399 DOI: 10.1038/srep26399
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