ProteoFluor™ Series
[Cancer cell detecting probe]
495-540 nm：Green
ProteoGREEN-gGlu (gGlu-HMRG) is a fluorescent probe to detect γ-glutamyl transpeptidase (GGT) activity. The probe irreversibly reacts with GGT which is highly expressed on the surface of cancer cells or cancer tissues, and then turns into green fluorescent materials. Since the green materials are rapidly taken into the cells, ProteoGREEN-gGlu can detect the cancer tissues which highly express GGT.
Available through Merck KGaA (Darmstadt, Germany) as:
SCT028 BioTracker™ 525 Green GGT Dye
| Code No. | Product Name | Size | Merck CAT No. |
Merck ( Millipore / Sigma Aldrich ) Product Name |
|---|---|---|---|---|
| GC801 | ProteoGREEN™-gGlu | 20 μg × 10 | SCT028 | BioTracker 525 Green GGT Dye |
ProteoGREEN™-gGlu
Product Information
| Product Name |
target | reaction | Absmax (nm) | FLmax (nm) | ε | Φ |
| ProteoGREEN-gGlu | γ-glutamyltransferase (γ-GT, GGT) | irreversible | 500 | 524 | 57,000 | 0.81 |
※These data are after reaction with GGT because ProteoGREEN-gGlu has almost no fluorescence before reacting with GGT.
Absorbance/fluorescence spectra of ProteoGREEN-gGlu upon reaction with and without GGT in PBS (pH 7.4)
ProteoGREEN™-gGlu
A cell imaging example using ProteoGREEN-gGlu
These images are taken 15 minutes after Hela (cell line from human uterocervical cancer, left) cells and HUVEC (human umbilical vein endothelial cell, right) cells were treated with 1 µM ProteoGREEN-gGlu in PBS at 37°C. Green color represents the fluorescence of ProteoGREEN-gGlu and the blue represents the fluorescence of Hoechst 33342, a nuclei marker.
Note that the probe also stains cells without GGT activity, because ProteoGREEN-gGlu-derived fluorophore generated on the GGT-positive cell surface diffuses through the medium and can stains nearby GGT-negative cells. Therefore, this probe is not suitable for distinguishing each cell properties in the mixture of GGT-positive and negative cells.
Cells with enhanced GGT-activity
Cells with little increase in GGT activity
ProteoGREEN™-gGlu
Animations of ProteoGREEN-gGlu
H. Ueo, I. Minoura, H. Ueo, A. Gamachi, Y. Kai, Y. Kubota, T. Doi, M. Yamaguchi, T. Yamashita, H. Tsuda, T. Moriya, R. Yamaguchi, Y. Kozuka, T. Sasaki, T. Masuda, Y. Urano, M. Mori, K. Mimori (2022)
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BJS Open 108: e340–e342 DOI: 10.1093/bjs/znab265
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A. Mochida, F. Ogata, Y. Maruoka, . Nagaya, R. Okada, F. Inagaki, D. Fujimura, P. L. Choyke, H Kobayashi (2018)
Oncotarget 9: 36039-36047 DOI: 10.18632/oncotarget.26320
R. Hino, N. Inoshita, T. Yoshimoto, M. Ogawa, D. Miura, R. Watanabe, K. Watanabe, M. Kamiya, Y. Urano (2018)
Thyroid Res. 11: 16, DOI: 10.1186/s13044-018-0060-y
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Oral Oncol. 78: 1-7 DOI:10.1016/j.oraloncology.2017.12.006
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BMC Cancer 17: 316 DOI:10.1186/s12885-017-3301-x
Y. Miyata, T. Ishizawa, M. Kamiya, S. Yamashita, K. Hasegawa, A. Ushiku, J. Shibahara, M. Fukayama, Y. Urano, N. Kokudo (2017)
Sci. Rep. 7: Article number: 3542 DOI:10.1038/s41598-017-03760-3
A. Behrooz, P. Waterman, K. O. Vasquez, J. Meganck, J. D. Peterson, I. Faqir, J. Kempner (2017)
Opt. Lett. 42: 2964-2967 DOI:10.1364/OL.42.002964
Y. Nakamura, T. Harada, T. Nagaya, K. Sato, S. Okuyama, P. L. Choyke, H. Kobayashi (2016)
Oncotarget 7: 51124–51137 DOI:10.18632/oncotarget.9898
T. Mizushima, S. Ohnishi, Y. Shimizu, Y. Hatanaka, K. C. Hatanaka, H. Hosono, Y. Kubota, M. Natsuizaka, M. Kamiya, S. Ono, A. Homma, M. Kato, N. Sakamoto, Y. Urano (2016)
BMC Cancer 16: 411 DOI:10.1186/s12885-016-2421-z
H. Ueo, Y. Shinden, T. Tobo, A. Gamachi, M. Udo, H. Komatsu, S. Nambara, T. Saito, M. Ueda, H. Hirata, S. Sakimura, Y. Takano, R. Uchi, J. Kurashige, S. Akiyoshi, T. Iguchi, H. Eguchi, K. Sugimachi, Y. Kubota, Y. Kai, K. Shibuta, Y. Kijima, H. Yoshinaka, S. Natsugoe, M. Mori, Y. Maehara, M. Sakabe, M. Kamiya, J. W. Kakareka, T. J. Pohida, P. L. Choyke, H. Kobayashi, H. Ueo, Y. Urano, K. Mimori (2015)
Sci. Rep. 7: 12080 DOI:10.1038/srep12080
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Gut 62: 1179-1186 DOI:10.1136/gutjnl-2011-301795
M. Sakabe, D. Asanuma, M. Kamiya, R. J. Iwatate, K. Hanaoka, T. Terai, T. Nagano, Y. Urano (2013)
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Y. Urano (2012)
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