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ProteoFluor™ Series

ProteoGREEN™-gGlu

[Cancer cell detecting probe]

495-540 nm：Green

ProteoGREEN-gGlu (gGlu-HMRG) is a fluorescent probe to detect γ-glutamyl transpeptidase (GGT) activity. The probe irreversibly reacts with GGT which is highly expressed on the surface of cancer cells or cancer tissues, and then turns into green fluorescent materials. Since the green materials are rapidly taken into the cells, ProteoGREEN-gGlu can detect the cancer tissues which highly express GGT.

 

Available through Merck KGaA (Darmstadt, Germany) as:
SCT028 BioTracker™ 525 Green GGT Dye

Products

Code No. Product Name Size Merck CAT No. Merck ( Millipore / Sigma Aldrich )
Product Name
GC801 ProteoGREEN™-gGlu 20 μg × 10 SCT028 BioTracker 525 Green GGT Dye

Downloads

  • protocol

  • SDS

  • Product Information

    ProteoGREEN™-gGlu
    Product Information

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    Properties of ProteoGREEN-gGlu

    Product Name
    		 target reaction Absmax (nm) FLmax (nm) ε Φ
    ProteoGREEN-gGlu γ-glutamyltransferase (γ-GT, GGT) irreversible 500 524 57,000 0.81

    ※These data are after reaction with GGT because ProteoGREEN-gGlu has almost no fluorescence before reacting with GGT.

     

    Absobance Fluorescence Spectrum

     

    Absorbance/fluorescence spectra of ProteoGREEN-gGlu upon reaction with and without GGT in PBS (pH 7.4)

     

  • A cell imaging example using ProteoGREEN-gGlu

    ProteoGREEN™-gGlu
    A cell imaging example using ProteoGREEN-gGlu

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    A cell imaging example using ProteoGREEN-gGlu

    GGT activity detection of HeLa cells

    These images are taken 15 minutes after Hela (cell line from human uterocervical cancer, left) cells and HUVEC (human umbilical vein endothelial cell, right) cells were treated with 1 µM ProteoGREEN-gGlu in PBS at 37°C. Green color represents the fluorescence of ProteoGREEN-gGlu and the blue represents the fluorescence of Hoechst 33342, a nuclei marker.

    Note that the probe also stains cells without GGT activity, because ProteoGREEN-gGlu-derived fluorophore generated on the GGT-positive cell surface diffuses through the medium and can stains nearby GGT-negative cells. Therefore, this probe is not suitable for distinguishing each cell properties in the mixture of GGT-positive and negative cells.

     

    List of cell lines which application of ProteoGREEN-gGlu has been reported

    Cells with enhanced GGT-activity

    • Cell lines from human ovarian cancer: SHIN-3, SK-OV-3, OVCAR-3, OVCAR-4, OVCAR-5, OVCAR-8, A2780, A2780 PTX22, IGR-OV1, Hey-A8, CaOV3
    • Cell line from human lung cancer: A549
    • Cell line from human biliary cancer: HuCCT1
    • Cell line from human hepatic cancer: HepG2
    • Cell line from human uterocervical cancer: HeLa
    • Human breast cancer tissues

    Cells with little increase in GGT activity

    • HUVEC (Human Umbilical Vein Endothelial Cells)
    • SW782 (a non-cancerous cell line of human pre-adipocytes)
    • HEK293 cells (Human embryonic kidney 293 cells)

  • Animations of ProteoGREEN-gGlu

    ProteoGREEN™-gGlu
    Animations of ProteoGREEN-gGlu

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    Image animation of in vivo imaging

     

     

     

Reference

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