【Fluorescence probes for super-resolution imaging】
|A201-01||HaloTag SaraFluor 650B Ligand||15 nmol||For super-resolution live-cell imaging with HaloTag. (red excitation)|
|A208-01||SaraFluor 650B-NHS||100 μg||For labeling proteins or other amine-containing compounds. (red excitation)|
|A209-01||SaraFluor 650B-maleimide||100 μg||For labeling thiols of proteins or antibodies. (red excitation)|
|A218-01||SaraFluor 488B-NHS||100 μg||For labeling proteins or other amine-containing compounds. (blue excitation)|
The theoretical limit of the resolution of fluorescence microscopy imaging is about half of the light wavelength (200-400 nm). A number of new imaging methods called “superresolution fluorescence microscopy” exceeding this limitation have been developed. One of them, called single molecule localization microscopy (SMLM), is widely used. Developed exclusively for SMLM, SaraFluor B series* is a fluorescent probe that exhibits spontaneous blinking under physiological conditions. The lineup includes SaraFluor 650B (SF650B, HMSiR) which emits deep red light with red laser excitation, and SaraFluor 488B (SF488B, HEtetTFER) which emits green light with blue laser excitation. Target molecules can be labeled by HaloTag, NHS or maleimide.
* Sara means spacious, bright in Ainu language. Probes which exhibit spontaneous blinking are marked with letter B, first letter of the word “blinking”.
** These products are the same as those sold under the name of HMSiR labeling Goat IgG (whole) anti-mouse / rat / rabbit IgG H&L.
Conventional fluorescence and SMLM images of fixed A549 cell stained with anti-α-tubulin antibodies (DM1A 1/4000 dilution) and SaraFluor 488B labeled secondary antibody. The total internal reflection fluorescence microscopy images were taken using NIKON Ti, NIKON Apo TIRF100x (NA 1.49) and ImagEM (Hamamatsu Photonics) with 488 nm laser excitation at the Nikon Imaging Center at Hokkaido University. The SMLM image was processed using ImageJ and ThunderSTORM.
|Product name||Absmax (nm)||FLmax (nm)||ε||Φ|
Spontaneous blinking of SaraFluor B in PBS (pH 7.4). Measured intensity change in a region corresponding to one molecule from a fluorescence image obtained by total internal reflection fluorescence microscopy with 647 nm laser excitation (SaraFluor 650B, HEtetTFER).(It is impossible to compare two vertical axes because different optical measurement systems were used.)
S. Uno, M. Kamiya, A. Morozumi, Y. Urano (2018)
Chem. Commun. 54:102-105 DOI: 10.1039/c7cc07783a (SF488B, SF650B)
F-C. Chien, C-Y, Linb, G. Abrigo (2018)
Phys. Chem. Chem. Phys. 20:27245-27255 DOI:10.1039/C8CP02942C (SF650B)
M. Ovesný, P. Křížek, J. Borkovec, Z. Švindrych, G. M. Hagen. (2014)
Bioinformatics 30:2389-2390 DOI: 10.1093/bioinformatics/btu202 (ThunderSTORM)
S. Uno, M. Kamiya, T. Yoshihara, K. Sugawara, K. Okabe, M. C. Tarhan, H. Fujita, T. Funatsu, Y. Okada, S. Tobita, Y. Urano (2014)
Nat. Chem. 6:681-689 DOI: 10.1038/NCHEM.2002 (SF650B)
※SaraFluor 650B corresponds to HMSiR (compound name) in the reference.