NIRFluor™ series
[Near-infrared fluorophore for labeling]
750 nm<：NIR
Indocyanine-green conjugated with NHS that can be used for labeling primary amines, or with maleimide that can be used for labeling thiols. It can be used for labeling antibodies and other macromolecules.
Excitation/Emission maximum (nm): 790/830
Available only in Japan.
We are sorry but these products are discontinued.
| Code No. | Product Name | Size | Merck CAT No. |
Merck ( Millipore / Sigma Aldrich ) Product Name |
|---|---|---|---|---|
| A501-01 | ICG-NHS ester | 1 mg | ||
| A501-05 | ICG-NHS ester | 1 mg × 5 | ||
| A501-10 | ICG-NHS ester | 1 mg × 10 |
Prepare purified antibody (or other proteins). If you intended to crude protein sample, we recommend to purify protein using either affinity column, ultrafilteration or gel filteration before the labeling to increase labeling efficiency. In addition, avoid to use Tris-buffer, because Tris has primary amine and it strongly inhibit the reaction of NHS to the target protein.
In some cases, reaction at 4oC for overnight gives higher degree of labeling compared with reaction at 37oC for 1 hour.
Molar extinction coefficient of protein at 280 nm can be calculated by the methods of Gill and von Hippel (1989) Analytical Biochemistry, 182: 319-326 and Anthis and Clore (2013) Protein Science 22:851-858.
You may find Web services to obtain them in the following sites. Goryo Chemical do not support the usage of these sites and use them according to the Terms and Conditions in each sites.
http://protcalc.sourceforge.net/
http://web.expasy.org/protparam/
http://nickanthis.com/tools/a205.html
The reason to recommend this buffer is that the reaction of NHS ester and primary amines is more efficient at alkaline pH. Instead, you can use other alkaline buffers such as HEPES/phosphate/borate buffers. Avoid to use Tris buffer because it has primary amines and inhibit the reaction of NHS with the target molecules.
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