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StemFluor™ Series

Kyoto Probe (KP-1)

[Human stem (iPS/ES) cells detecting probe]

495-540 nm：Green

  • Able to distinguish human iPS cells and/or human ES cells from differentiated cells
  • Usable for flow cytometry or live cell imaging
  • Staining while culturing

 

Available through Merck KGaA (Darmstadt, Germany) as:
SCT029 BioTracker™ 529 Green Pluripotent Stem Cell Dye

 

Products

Code No. Product Name Size Merck CAT No. Merck ( Millipore / Sigma Aldrich )
Product Name
GC7001-01 Kyoto Probe 1 (KP-1) 10 μg × 5 SCT029 BioTracker 529 Green Pluripotent Stem Cell Dye
GC7001-02 Kyoto Probe 1 (KP-1) 10 μg × 10

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  • Product Information

    Kyoto Probe (KP-1)
    Product Information

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    Properties of KP-1

    名称 Cell permeability
    		 Absmax (nm) FLmax (nm) Φ
    Kyoto Probe 1 (KP-1) yes 515 529 0.45

    Spectra

    Fluorescent feature of KP-1. (Cited from N. Hirata et al. 2014 Cell Rep. 6: 1165-1174)

  • KP-1: Imaging examples and mechanism of staining

    Kyoto Probe (KP-1)
    KP-1: Imaging examples and mechanism of staining

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    KP-1: Imaging examples and mechanism of staining

    An example of human iPS cells stained with KP-1

    A human iPS cell colony on feeder cells stained with KP-1 (left, a bright-field image; right, a fluorescent image)  (Hirata, N. et al. 2014 Cell Rep.6: 1165-1174)

     

    The mechanism of stem-cell specific staining with KP-1

     

    KP-1 is a cell-permeable fluorophore which is incorporated into cells. However, differentiated human cells export it through ABC transporters and thus, differentiated cells are not stained. In contrast, because of low expression levels of ABCB1 and ABCG2 in human stem cells, KP-1 accumulates in mitochondria of human stem cells. As a consequences, human stem cells can be distinguished with differentiated cells by the staining of KP-1.  (N. Hirata, et al., 2014, Cell Rep. 6: 1165-1174)

     

    Examples of human ES cells stained with KP-1

    As well as human iPS cells  (C, F),  embryonic stem cells (ES cells,  D, G) are stained. In a partially differentiated cell cluster, only a part of the cell cluster was stained  (E, H).  (Figures from Hirata, N. et al. 2014 Cell Rep. 6: 1165-1174 )

FAQ

  • Q Does KP-1 have cytotoxicity?
    A

    We did not observe cytotoxic effects of KP-1 in 1μM, a concentration typically used. We tested 48-hour culture in the presence of 1 μM KP-1 and found it to not affect the cell viability.

  • Q KP-1 can be applied to mouse-derived embryonic stem cells?
    A

    In human stem cells, weak expression of ABCB1 and ABCG2 (ABC transporters), by those transporters KP-1 are excreted out, have been confirmed. In contrast expression profile of these transporters might be different in mouse and in other mammalian cells. Therefore you cannot expect the efficacy of KP-1 in cells other than human cells. We recommend to test the reagent on the cells beforehand.

  • Q My question is not in this FAQ list.....
    A

    Please also refer the frequently asked questions on fluorescent probes

     

Reference

C. Miyagi‑Shiohira, I. Saitoh, M. Watanabe, H. Noguchi (2020)
Sci. Rep. 10:18084 DOI: 10.1038/s41598-020-75016-6

D. Mao, S. Ando, S. Sato, Y. Qin, N. Hirata, Y. Katsuda, E. Kawase, T. F. Kuo, I. Minami, Y. Shiba, K. Ueda, N. Nakatsuji, M. Uesugi (2017)
Angew. Chem. Int. Ed. Engl. 56: 1765-1770 DOI:10.1002/anie.201610284

T. F. Kuo, D. Mao, N. Hirata, B. Khambu, Y. Kimura, E. Kawase, H. Shimogawa, M. Ojika, N. Nakatsuji, K. Ueda, M. Uesugi (2014)
J. Am. Chem. Soc. 136: 9798-9801 DOI:10.1021/ja501795c

N. Hirata, M. Nakagawa, Y. Fujibayashi, K. Yamauchi, A. Murata, I. Minami, M. Tomioka, T. Kondo, T. F. Kuo, H. Endo, H. Inoue, S. Sato, S. Ando, Y. Kawazoe, K. Aiba, K. Nagata, E. Kawase, Y. T. Chang, H. Suemori, K. Eto, H. Nakauchi, S. Yamanaka, N. Nakatsuji, K. Ueda, M. Uesugi (2014)
Cell Rep. 6: 1165-1174 DOI:10.1016/j.celrep.2014.02.006

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