[Sulfane sulfur-detecting fluorescence probe]
SSip-1 DA

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CodeProductSizePrice
A402-1SSip-1 DA60 nmol ×3$498.00

Intracellularly abundant sulfane sulfur species including persulfide (R-S-SH), polysulfide (R-S-Sn-S-R) and polysulfide (H2Sn) play important roles to maintain intracellular reducing environments. SSip-1 DA is a FRET-based fluorescence probe to specifically detect the intracellular sulfane sulfur species. It shows only week fluorescence without sulfane sulfur, and reversibly fluoresces in response to micromolar concentrations of sulfane sulfur. Its reaction is highly specific and has minimal to no reactivity with H2S, cysteine residues, and sulfur oxides. SSip-1 DA, a cell permeable diacetylated (DA) form of SSip-1 is hydrolyzed by intracellular esterases to generate SSip-1 which retains within the cells. Thus it is suitable to monitor intracellular concentration of sulfane sulfur via live-cell imaging.

Data

producttargetCell permeabilityfluorescenceAbsmax(nm)Emmax(nm)
SSip-1 DAsulfane sulfurYes (DA)reversible (in the presence of GSH)495525

Absorption and emission spectra

Left, absorption spectra of 5 μM of the reagent, and right, fluorescence spectra excited at 470 nm.

Imaging example

Intracellular sulfane sulfurwasimaged with SSip-1 DA, before (left) and after (right) a stimulation with 5 μM Na2S4 in A549 cells. Cells weretreated with 10 μM SSip-1 DA (containing 1 mg/mL BSA as additives)for 1 hour at 37oC, 5% CO2. After washing, the cells in HBSS were observed under a fluorescence microscopeusing 460―500 nm excitation filter and 512―542 fluorescence filter. Grayscale images of DIC and green pseudocolor images of fluorescence were overlaid. A549 cell was provided from JCRB cell bank. Scale bar, 50 μm. Please refer to application note for details regarding the observation conditions and other applications.

References

D. Ezeriņa, Y. Takano, K. Hanaoka, Y. Urano, T. P. Dick (2018) Cell Chem. Biol.25: 447-459.e4 DOI: 10.1016/j.chembiol.2018.01.011

R. Miyamoto, S. Koike, Y. Takano, N. Shibuya, Y. Kimura, K. Hanaoka, Y. Urano, Y. Ogasawara, H. Kimura (2017)Sci. Rep., 7: 45995 DOI: 10.1038/srep45995

Y. Takano, K. Hanaoka, K. Shimamoto, R. Miyamoto, T. Komatsu, T. Ueno, T. Terai, H. Kimura, T. Nagano, Y. Urano (2017)
Chem. Commun., 53: 1064-1067 DOI: 10.1039/C6CC08372B

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