AcidiFluor™ ORANGE



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GC301AcidiFluor™ ORANGE10μg×20$680.00
  • High S/N ratio
  • Orange color fluorescence and usable for multicolor imaging
  • Great photostability

AcidiFluor™ ORANGE is a fluorescence imaging probe which enhances fluorescence dramatically in acidic environments. This probe can stain acidic organelles such as lysosome, late endosome and granule selectively. Its excellent selectivity enables to detect acidic environment. Namely, in the condition of pH5.0, identical to the environment of acidic organelles, fluorescence intensity is 50-fold or more compared to physical pH 7.4. AcidiFluor™ ORANGE shows great photostability against irradiating excitation light. As it emits orange color fluorescent by excitation at 532nm or 514 nm, multicolor imaging is possible by combining with blue fluorescence (CFP, Hoechst, etc.), green fluorescence (GFP, fluorescein, etc.) and near-infrared fluorescence. AcidiFluor™ ORANGE can be used for detecting acidic organelles, observing granule release, imaging of endocytosis/exocytosis and so on.

Feature of AcidiFluor™ ORANGE

λabs 535 nm
λfl 560 nm

pKa 5.1

High S/N ratio

Figure 1. Fluorescence spectra of AcidiFluor Orange as a function of pH.

Fluorescence spectra of AcidiFluor Orange was measured in phosphate buffer pH 5.0 or pH 7.4, respectively. pH 5.0 buffer corresponds to the condition in the acidic organelles, and pH 7.4 corresponds to the physiological cytoplasmic conditions. Fluorescent intensity of AcidiFluor Orange at pH 5.0 increased 50 fold to that in the pH 7.4 buffer. ( λex 532 nm / λem 568 nm )

Emits orange color fluorescence, usable for multicolor imaging

Fig.2 Example of multicolor imaging using HeLa cell

HeLa cell expressed Mitochondria-GFP was stained multiplicity with AcidiFluor™ ORANGE and Hoechst33342. Lysosomes were stained orange with AcidiFluor™ ORANGE, nucleuses were stained blue with Hoechst33342 and Mitochondria were stained green with GFP. As shown in Fig.2, AcidiFluor™ ORANGE can be useful for multicolor imaging

Great photostability

Fig.3 Comparison to competitor’s products by photocleaching test

Samples were continuously illuminated for 180 seconds and viewed on a confocal microscopy. Although LysoTracker® Green DND-26 and LysoTracker® Red DND-99 discolored dramatically, fluorescence of AcidiFluor™ ORANGE remained. LysoSensor™ Green DND-189 was not suitable for continuous observation because fluorescence leaked to cytoplasm by excitation light irradiation.
Goryo Chemical Company commercialized AcidiFluor™ ORANGE under the guidance of Prof. Kenzo Hirose (Professor of Graduate School of Medicine, The University of Tokyo, Department of Neurobiology). AcidiFluor™ ORANGE was licensed from Tokyo University. The development of this product was supported by JST (Japan Science and Technology Agency) program “Development of Systems and Technology for Advanced Measurement and Analysis”.


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”Watanabe R, Soga N, Fujita D, Tabata KV, Yamauchi L, Kim SH, Asanuma D, Kamiya M, Urano Y, Suga H and Noji H. Nature Communications 2014 Jul24; 5, Article number: 4519 doi:10.1038/ncomms5519 “Arrayed lipid bilayer chambers allow single-molecule analysis of membrane transporter activity”Asanuma D, Takaoka Y, Namiki S, Takikawa K, Kamiya M, Nagano T, Urano Y & Hirose K. Acidic-pH-Activatable Fluorescence Probes for Visualizing Exocytosis Dynamics. Angew Chem Int Ed 2014, doi:10.1002/anie.201402030.

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