This reagent is an acidic pH indicator, AcidiFluor ORANGE fused with HaloTag® ligand, which forms covalent bond with HaloTag. It shows quite weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH.
Since it is cell impermeable reagent, it an be used to label membrane-associated HaloTag fused proteins and monitor the repetitive endocytosis and exocytosis cycle of the target proteins.
HaloTag® is a registered trade mark of Promega Corporation. Goryo Chemical, Inc. produces HaloTag products under the license of Promega Corporation.
Available through Merck KGaA (Darmstadt, Germany) as:
SCT212-30nmol HaloTag® AcidiFluor™ORANGE Ligand
SCT212-60nmol HaloTag® AcidiFluor™ORANGE Ligand