[Fluorophores for labeling]
STELLA Fluor™ series

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CAT. No. Wavelength (nm) Reactive group Amount Price
ST1003-10 488 NHS 5 nmol x 5
$350.00
ST1003-11 1 mg $498.00
ST1003-15 5 mg $2,210.00
ST1003-21 maleimide 1 mg $498.00
ST1003-25 5 mg
$2,210.00
ST1003-31 COOH 1 mg
$448.00
ST1003-35 5 mg
$1,990.00
ST1003-41 NH2 1 mg
$548.00
ST1003-45 5 mg
$2,430.00
ST1006-10 600 NHS 5 nmol x 5 $ 350.00
ST1006-11 1 mg $498.00
ST1006-15 5 mg
$2,210.00
ST1006-21 maleimide 1 mg
$498.00
ST1006-25 5 mg
$2,210.00
ST1006-31 COOH 1 mg
$448.00
ST1006-35 5 mg
$1,990.00
ST1006-41 NH2 1 mg
$548.00
ST1006-45 5 mg
$2,430.00
ST1008-10 650 NHS 5 nmol x 5 $350.00
ST1008-11 1 mg
$498.00
ST1008-15 5 mg
$2,210.00
ST1008-21 maleimide 1 mg
$498.00
ST1008-25 5 mg
$2,210.00
ST1008-31 COOH 1 mg
$448.00
ST1008-35 5 mg
$1,990.00
ST1008-41 NH2 1 mg
$548.00
ST1008-45 5 mg
$2,430.00
ST1010-10 700 NHS 5 nmol x 5 $350.00
ST1010-11 1 mg
$498.00
ST1010-15 5 mg
$2,210.00
ST1010-21 maleimide 1 mg
$498.00
ST1010-25 5 mg
$2,210.00
ST1010-31 COOH 1 mg
$448.00
ST1010-35 5 mg
$1,990.00
ST1010-41 NH2 1 mg
$548.00
ST1010-45 5 mg
$2,430.00
ST1011-10 720 NHS 5 nmol x 5 $350.00
ST1011-11 1 mg
$498.00
ST1011-15 5 mg
$2,210.00
ST1011-21 maleimide 1 mg
$498.00
ST1011-25 5 mg
$2,210.00
ST1011-31 COOH 1 mg
$448.00
ST1011-35 5 mg
$1,990.00
ST1011-41 NH2 1 mg $548.00
ST1011-45 5 mg $2,430.00

STELLA Fluor series is a product line of bright fluorophores, which are suitable for labeling proteins (e.g., antibodies) and other macromolecules. These products are also used for labeling, marking or to evaluate physical properties. Among them, STELLA Fluor 650, 700, and 720, derivatives of silicone rhodamine, are uniquely bright and photostable fluorophores in far-red to near-infrared wavelength range.

N-hydroxysuccinimide (NHS) esters quickly form a covalent bond with primary amine just by mixing. NHS esters are widely used for labeling antibodies and macromolecules. On the other hand, maleimides (MLI) which forms a covalent bond with thiols (R-SH) are used to specifically label cysteine residues. The fluorophores with amino groups (-NH2) and carboxyl groups (-COOH) are used for labeling using crosslinkers, used as a material for chemical synthesis.

Table 1 : Properties of STELLA Fluor

Name Excitation max. (nm) Emission max. ( nm) Molar extinction coefficient (M-1cm-1) Quantum Yield Corresponding dyes
STELLA Fluor 488 494 514 0.82× 105 0.91 FITC, BODIPY FL, Alexa Fluor 488, ATTO 488
STELLA Fluor 600 597 607 1.3× 105 0.48 Cy3.5, Texas Red, DyLight 594, Alexa Fluor 594
STELLA Fluor 650 646 660 1.1 × 105 0.31 Alexa Fluor 647, ATTO 647N, Cy5, DyLight 650
STELLA Fluor 700 691 712 1.0 × 105 0.12 Alexa Fluor 700, ATTO 700
STELLA Fluor 720 721 740 1.6× 105 0.05 Cy7, DY-730, ATTO 725

wavelength

Figure 1: Spectra of STELLA Fluor series (dot lines : absorption spectra, solid lines : emission spectra)

steela

Figure 2. A comparison of photobleaching property of STELLA Fluor 650

Fixed cells were stained with secondary antibodies labeled with STELLA Fluor 650 or another corresponding fluorophore, and were observed by fluorescence microscopy. Photobleaching was observed under the same imaging conditions including light intensity, fluorescent filters, exposure, and camera gain.

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