Contract Uptake Assay Systems of Fluorescent-Labeled Substances
Out of stock
– Quantitating the activity of uptaking of substance by cells using fluorescence
– Easy to screening many samples by 96well plate―
We label the substance with fluorescence and produce your own assay plate. Drugs which enhance/atteuate activity of uptake of substance by cells are quantified simply. We label your substance with fluorescence your request.
- Example of exam：Quantitation uptaking of TAMRA labeled amyloid-β by Microglia cell
- Label amyloid-β with TAMRA by solid-phase synthesis.
- Oligomerize TAMRA labeled amyloid-β see detail and coated on 96 well plate.
- Seed microglia cell.
- Incubate for 24 hours, collect culture supernatant and measure fluorescence intensity(Ex.:555nm, Em.:585nm). Control experiment is performed in the well with no cell.
TAMRA labeled amyloid-β oligomer were incorporated by Microglia cell and after 24 hours, collected supernatants were measured fluorescence intensity. It is suggested that microglia cells incorporated TAMRA labeled amyloid-β and released to culture supernatant.
Microglial cells was seeded on the plate which was coated with TMR-βamyloid oligomer and stimulated with various drugs. After 24 hours, the supernatant was collected and fluorescence intensity was measured. The Graph indicates relative change of uptake activity (additive-free Control is assumed 1) of TMR-β amyloid oligomer by each drugs.
Goryo Chemical, Inc. Custom Assay System survice
|Please provide us purified substance which is incorporated by cells.|
|We label the substance with fluorescence you requested and purify them.|
|We coat 96well plate with fluorescence labeled substance.|
|Delivery||We delivery assay plate with report.|
Price and delivery time
|Fluorescence labeld assay plate||1plate||Contact us||About 4 weeks|