ICG-NHS ester labeling kit

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Code No. Product Size Price Application & Note
A501-21 ICG-NHS ester labeling kit 5 times $250 ICG-NHS ester labeling kit provide a convenient means for labeling fluorescent probe to a small amount of antibody or protein (20–100 µg), and supply everything needed for labeling reactions. Exmax / Emmax (nm): 790/830

Indocyanine Green Labeling Kit

Indocyanine Green-NHS (succinimidyl ester) is near-infrared fluorescent probe that binds to amino acid residues without any condensing agents. Not only proteins or antibodies but also oligo-nucleotide with amino acid at the end can be labeled. This kit contains reagents and tools that required for the labeling for five times with Indocyanine Green-NHS and purification. One package of Indocyanine Green-NHS is optimum for the labeling of 300 μg of proteins, such as IgG.

Indocyanine Green Labeling Kit
Contents of the kit
  • Indocyanine Green-NHS× 5
  • Dimethylsulfoxide500μL × 1
  • Reaction Buffer5mL × 1
  • Washing Buffer10mL × 1
  • Spin column for ultrafiltration× 5

Labeling of antimicrobial peptide with NHS (Example)

Reference

Boudewijn E. Schaafsma, MD, J.Sven D. Mieog, MD, Merlijn Hutteman, MSc, Joost R. van der Vorst, MD, Peter J.K. Kuppen, PhD, Clemens W.G.M. Löwik, PhD, John V. Frangioni, MD, PhD, Cornelis J.H. van de Velde, MD, PhD, and Alexander L. Vahrmeijer, MD, PhD,* J Surg Oncol. 2011 Sep1; 104(3): 323-332., “The clinical use of indocyanine green as a near-infrared fluorescent contrast agent for image-guided oncologic surgery”

K. Sigmundsson, J. R.M. Ojala, M. K. Öhman, A. M. Österholm, A. Moreno-Moral, A. Domogatskaya, L. Y. Chong, Y. Sun, X. Chai, J. A. M. Steele, B. George, M. Patarroyo, A. S. Nilsson, S. Rodin, S. Ghosh, M. M. Stevens, E. Petretto, K. Tryggvason. (2018)
Matrix Biol.pii: S0945-053X(18)30052-0 DOI: 10.1016/j.matbio.2018.03.018

D. Matsuoka, H. Watanabe, Y. Shimizu, H. Kimura, Y. Yagi,R. Kawai, M. Ono, H. Saji (2018)
Bioorg. Med. Chem. In press DOI: 10.1016/j.bmc.2018.03.015

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