POLARIC® Series

POLARIC®-500BCS

[Bacterial membrane detecting probe]

Others

POLARIC-BCS  is a solvatochromic dye for staining bacteria. It stains both gram-positive and gram-negative bacteria cell membranes.

For laser excitation, 488 nm or 514 nm is applicable.

 

Products

Code No. Product Name Size Merck CAT No. Merck ( Millipore / Sigma Aldrich )
Product Name
GC102 POLARIC®-500BCS 5 μg × 10
GC1021 POLARIC®-500BCS 5 μg × 5
  • Product Information

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    Properties of POLARIC-500BCS

    Product name
    target reaction Absmax (nm)* FLmax (nm)*
    POLARIC-500BCS solvent polarity reversible 480 592

    *Because of low water solubility of POLARIC-500BCS, it is a value determined in aqueous medium containing SDS. It changes depending on the solvent polarity.

    An example spectra in bacterial cell membrane

     

    For laser excitation, 488 nm or 514 nm is applicable.

  • Imaging examples with POLARIC-500BCS

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    Imaging examples with POLARIC-500BCS

     

     

    Salmomella typhimurium stained with POLARIC-500BCS  were imaged wth structured illumination microscopy (Nikon, N-SIM). Ex: 488 nm, Em: 510-550 nm. Membrane structure of the bactria were clearly observed.

     

FAQ

  • Q Can I selectively stain bacterial membranes with POLARIC-500BCS?
    A

    No. POLARIC-500BCS also stains eukaryotic cells including mammalian culured cells. You can not selectively stain prokaryotes.

     

  • Q In what situation does POLARIC change the color?
    A

    It is reported that the fluorescent wavelength of POLARIC is changed by the composition of lipid (Chem. Lett. 2011, 4, 989-991). On the other hand, POLARIC show no significant difference of fluorescence wavelength by pH changes.

Reference

Y. Kawasaki, T. Endo, A. Fujiwara, K. Kondo, M. Katahira, T. Nittami, M. Sato, M. Takeda (2016)
Int. J. Biol. Macromol. 95: 1280-1288 DOI:10.1016/j.ijbiomac.2016.11.025