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BacteriTrace Vanco™ (Vancomycine ICG probe)

Content and storage

Material Amount Storage Stability
Vanco-ICG 0.1 mg
  • -20 °C
  • Dessicate
  • Protect from light
When stored as directed, reactive probes are stable for at least 3 months
1 mg
5 mg

General Specifications

Color: Green powder

Label: ICG

Product Size: 1 kit

Detection Method: Fluorescence, optoacoustic

Excitation Class: Near infrared, NIR

Excitation/Emission (nm): 790/830

Molecular Weight: 2474.58 g.mol-1

Solubility: DMSO, DMF

Shipping Condition: Dry Ice

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Introduction

Intrace Medical has developed a NIR-fluorescent probe targeting Gramm-positive bacteria that potentially allows in vivo imaging of bacteria infection.

Gram-positive bacteria are differentiated from Gram-negative bacteria based on the structure of their cell walls. In Gram-positive bacteria, the cell wall is composed of a thick peptidoglycan layer.1 Many antibiotics have been established to treat Gram-positive infections. Vancomycin is a commonly used glycopeptide antibiotic that acts on inhibiting bacterial cell wall synthesis. In order to create an imaging agent, we attach the near infra-red fluorescence imaging dye Indocyanine Green (ICG) to vancomycin. 

ICG is approved by the United States Food and Drug administration (FDA) for ophthalmologic angiography to determine cardiac output and liver blood flow and function. This dye is also used in cancer patients for the detection of solid tumors, localization of lymphnodes, and for angiography during reconstructive surgery, visualization of retinal and choroidal vasculature, and photodynamic therapy.[i]2-3 In cancer diagnostics and therapeutics, ICG could be used as both an imaging dye and a hyperthermia agent.

ICG is a tricarbocyanine-type dye with NIR-absorbing properties (peak absorption around 800 nm) and little absorption in the visible range thus exhibit low autofluorescence, tissue absorbance, and scatter at NIR wavelengths (700-900 nm). 

Guidelines for use

Immediately before use, dissolve BacteriTrace Vanco™ in dimethylsulfoxide (DMSO). Once reconstituted in DMSO, this reactive probe solution is somewhat unstable and should be diluted with a buffer solution to the final DMSO content 5%. 

The working dose of the probe for in vivo imaging strongly depends on the model used but it is recommended to start with 1-5 mg kg-1.

Examples are described elsewhere4

References
  1. J. Van Heijenoort, Glycobiology 2001, 11, 25R – 36R
  2. Schaafsma B.E. The clinical use of indocyanine green as a near-infrared fluorescent contrast agent for image-guided oncologic surgery. J. Surg. Oncol. 2011, 104, 323-332
  3. Saxena V, Sadoqi M, Shao J. Degradation kinetics of indocyanine green in aqueous solution. J. Pharm. Sci. 2003, 92, 2090–7
  4. Van Oosten, M., Schäfer, T., Gazendam, J. A. C., Ohlsen, K., Tsompanidou, E., de Goffau, M. C., Harmsen, H. J. M., Crane, L. M. A., Lim, E., Francis, K. P., Cheung, L., Olive, M., Ntziachristos, V., van Dijl, J. M.,  van Dam, G. M. Real-Time in vovo Imaging of Invasive- and Biomaterial-Associated Bacterial Infections Using Fluorescently Labeled Vancomycin. Nature Comm. 2013, 4, 1-8

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