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[Detection and measurement of hydrogen peroxide] HYDROP™ , HYDROP-EX™

HYDROP is a fluorescent probe for specific detection of hydrogen peroxide (H2O2). It has low reactivity with other reactive oxygen species (ROS). HYDROP is used for live cell imaging. HYDROP-EX is for detection and quantification of hydrogen peroxide in solutions.

Features

  • It fluoresces upon reaction with H2O2, but does not react with other ROS such as hydroxyradical (OH), superoxide (O2-・), hypochlorous acid (HOCl), singlet oxygen (1O2), and nitric oxide (NO). 
  • Cell-permeable HYDROP is a diacetylated form of HYDROP-EX. Initially, HYDROP has low reactivity with H2O2 but it is quickly hydrolyzed by intracellular esterases to generate reactive and cell impermeable HYDROP-EX. Thus generated HYDROP-EX retains within a cell and fluoresces upon reaction with H2O2.

HYDROP, HYDROP-EX

Specifications

Name Excitation max (nm) Emission max
(nm)
Cell permeability Target Photo
stability
brightness
HYDROP 492 516 yes (DA) H2O2 low high
HYDROP-EX no

Refer the Application Note of HYDROP-EX for the detailed reactivity data.

Imaging example

Imaging example

An increase of H2O2 was observed after induction of autophagy to HeLa cells by starvation (top). In contrast, addition of antioxidant (N-acetyl cysteine, NAC) decreases the production of H2O2 (bottom).

Refer the Applicaiton Note of HYDROP for the details of the observation and other example applications.

References
T. Yamamoto, H. Nakano, K. Shiomi, K. Wanibuchi, H. Masui, T. Takahashi, Y. Urano, T. Kamata (2018)
Biol. Pharm. Bull. (in press) DOI: 10.1248/bpb.b17-00804
M. Abo, E. Weerapana (2018)
Antioxid. Redox. Signal. (in press)  DOI: 10.1089/ars.2017.7408
K. Tomita, Y. Kuwahara, Y. Takashi, T. Tsukahara, A. Kurimasa, M. Fukumoto, Y. Nishitani, T. Sato (2017) 
Biochem. Biophys. Res. Commun. 490: 330-335 DOI: 10.1016/j.bbrc.2017.06.044
J. L. Kolanowski, A. Kaur, E. J. New (2016)
Antioxid. Redox. Signal. 24:713-30  DOI:10.1089/ars.2015.6588
H. Guo, H. Aleyasin, B. C. Dickinson, R. E. Haskew-Layton, R. R. Ratan (2014)
Cell Biosci. 4:64  DOI:10.1186/2045-3701-4-64
M. Abo, Y. Urano, K. Hanaoka, T. Terai, T. Komatsu, T. Nagano (2011)
J. Am. Chem. Soc., 133:10629-10637 DOI: 10.1021/ja203521e
Price
Code Product Size Price Protocol SDS
GC3007-01 HYDROP 30 nmol×3 $398 Download
215KB
Download
258KB
GC3008-01 HYDROP-EX 30 nmol×3 $398 Download
204KB