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[Cancer cells detection probe] ProteoGREEN™-gGlu

– For specific detection of cancer cells –

  • Cancer cells specific
  • High S/N ratio
  • GGT (γ-glutamyltranspeptidase)-activatable fluorescent probe

ProteoGREEN™-gGlu is cancer-selective fluorescent imaging probe. This probe is rapidly activated by g-glutamyltranspeptidase (GGT), which is known to be tumor-associated enzyme, to give highly fluorescent compound.
The membrane-permeable ProteoGREEN™-gGlu is applicable to live cell or in vivo imaging of cancer cells.

ProteoGREEN™-gGlu has almost non-fluorescent in the absence of GGT. Treatment of ProteoGREEN™-gGlu with GGT results in a fluorescence turn-on with high S/N ratio.

in vivo imaging

 

Live cell imaging

Application

  • For fluorescence imaging of cancer cells

The feature of ProteoGREEN™-gGlu

λabs 496 nm

λFl 525 nm

Figure 1. The reaction mechanism of ProteoGREEN™-gGlu and GGT
Figure 1. The reaction mechanism of ProteoGREEN™-gGlu and GGT

Figure 2. The fluorescent spectra of ProteoGREEN™-gGlu after treated with GGT
Figure 2. The fluorescent spectra of ProteoGREEN™-gGlu after treated with GGT

ProteoGREEN™-gGlu was treated with GGT for 30 min at 37℃. After reacted, the fluorescence intensity markedly increased at 525 nm (S/N ratio).

Figure 3. Live cell imaging of cancer cells or HUVEC cells
Figure 3. Live cell imaging of cancer cells or HUVEC cells

Each cells were treated with ProteoGREEN™-gGlu (2 µM) for 1 h. The reaction was completely inhibited by treatment with GGT selective inhibitor.

Reference
Yuko Nakamura, Toshiko Harada, Tadanobu Nagaya, Kazuhide Sato, Shuhei Okuyama, Peter L. Choyke, Hisataka Kobayashi (2016)
Oncotarget. 7(32):51124-51137.
Fluorescent imaging of superficial head and neck squamous cell carcinoma using a
γ-glutamyltranspeptidase-activated targeting agent: a pilot study
Takeshi Mizushima, Shunsuke Ohnishi, Yuichi Shimizu, Yutaka Hatanaka, Kanako C. Hatanaka, Hidetaka Hosono, Yoshimasa Kubota, Mitsuteru Natsuizaka, Mako Kamiya, Shouko Ono, Akihiro Homma, Mototsugu Kato, Naoya Sakamoto, and Yasuteru Urano
BMC Cancer, 2016, 16, 411.  
Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green.
Ueo H, Shinden Y, Tobo T, Gamachi A, Udo M, Komatsu H, Nambara S, Saito T, Ueda M, Hirata H, Sakimura S, Takano Y, Uchi R, Kurashige J, Akiyoshi S, Iguchi T, Eguchi H, Sugimachi K, Kubota Y, Kai Y, Shibuta K, Kijima Y, Yoshinaka H, Natsugoe S, Mori M, Maehara Y, Sakabe M, Kamiya M, Kakareka JW, Pohida TJ, Choyke PL, Kobayashi H, Ueo H, Urano Y, Mimori K.
Sci Rep., 2015, 5, 12080. 
Rational Design of Highly Sensitive Fluorescence Probes for Protease and Glycosidase Based on Precisely Controlled Spirocyclization
Sakabe, M.; Asanuma, D.; Kamiya, M.; Iwatate, RJ.; Hanaoka, K.; Terai, T.; Nagano, T.;
Urano, Y.
J. Am. Chem. Soc., 2013 , 135, 409-414. 
Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe. 
Mitsunaga, M.; Kosaka, N.; Choyke, PL.; Young, MR.; Dextras, CR.; Saud, SM.;
Colburn ,NH.; Sakabe, M.; Nagano, T.; Asanuma, D.; Urano, Y.; Kobayashi, H. 

Gut, 2013, 62, 1179-1186.
Novel live imaging techniques of cellular functions and in vivo tumors based on precise design of small molecule-based ‘activatable’ fluorescence probes. 
Urano Y. 

Curr Opin Chem Biol.,2012, 16, 602-608.
Rapid cancer detection by topically spraying a γ-glutamyltranspeptidase-activated fluorescent probe. 
Urano, Y.; Sakabe, M.; Kosaka, N.; Ogawa, M.; Mitsunaga, M.; Asanuma, D.; Kamiya, M.; Young, M. R.; Nagano, T.; Choyke, P. L.; Kobayashi, H.
Sci. Transl. Med., 2011 , 129, 1-10.
ProteoGREEN™-gGlu is defined as gGlu-HMRG in the paper.
Price
Code No. Product Size Price Protocol MSDS
GC
801
ProteoGREEN
-gGlu
20μg×10 $ 650.00
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