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Diaminorhodamine-4M acetoxymethyl ester (DAR-4M AM)

Features

  • Permeable through the cell membrane and real time observation of NO inside tissues or cultured cells is available.
  • Low autofluorescence from the living sample because the reagent is excited by the light with long wave length.
  • High intensity of fluorescence at the wide range of pH (from pH 4 to 12).

Principle of the measurement

Amino groups of Diaminorhodamine react with NO, and it makes fluorescence of green light with wave length of 575 nm when it’s excited by the light with wavelength of 560 nm.

Diaminorhodamine-4M acetoxymethyl ester (DAR-4M AM)

Contents

DAR-4M AM1mg (in DMSO 0.32 mL)
C28H31IN4O5            Mw:630.47

Preparation of the reagent
Density of the provided sample is 5 mmol/L in DMSO. Dilute 500 times with neutral buffer before use.
Reference
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price
Code no. Product size Price Protocol MSDS
SK1006-01 Diaminorhodamine-4M acetoxymethyl ester(DAR-4M AM) 1mg $640 Download
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