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Diaminofluorescein-FM diacetate (DAF-FM DA)

Features

  • High fluorescence intensity even at low pH (around pH 6) conditions.
  • Permeable through the cell membrane and real time observation of NO inside tissues or cultured cells is available.
  • Long time localization inside the cell.
  • Easily observed by a fluorescence microscopy.
  • Low damage to the cells because the reagent is excited by the visible light.
  • High sensitivity and selectivity.

Principle of measurement

After the permeation through the cell membrane, DAF-FM DA is hydrolyzed by intracellular esterase to DAF-FM which is unpermeable through the cell membrane. Amino groups of DAF-FM capture NO, and the DAF-FM makes fluorescence of green light with wave length of 515 nm when it’s excited by the light with wavelength of 500 nm.

Contents

DAF-FM DA 1mg (in DMSO 0.4 mL)
C25H18F2N2O7             Mw:496.42

Preparation of  the reagent
Density of the provided sample is 5 mmol/L in DMSO. Dilute 500 times with neutral buffer before use.

Diaminofluorescein-FM diacetate (DAF-FM DA)

Reference
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price
Code no. Product size Price Protocol MSDS
SK1004-01 Diaminofluorescein-FM diacetate (DAF-FM DA) 1mg $506 Download
399KB
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175KB