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Diaminofluorescein-2 diacetate (DAF-2 DA)

Features

  • Permeable through the cell membrane and real time observation of NO inside tissues or cultured cells is available.
  • Long time localization inside the cell.
  • Easily observed by a fluorescence microscopy.
  • Low damage to the cells because the reagent is excited by the visible light.
  • High sensitivity and selectivity.

Principle of the measurement

After the permeation through the cell membrane, Diaminofluorescein-2 diacetate (DAF-2 DA) is hydrolyzed by intracellular esterase to Diaminofluorescein-2 (DAF-2) which is unpermeable through the cell membrane. Amino groups of Diaminofluorescein-2 (DAF-2) capture NO, and the DAF-2 makes fluorescence of green light with wave length of 515 nm when it’s excited by the light with wavelength of 495 nm.

Contents

DAF-2 DA 1mg (in DMSO 0.45mL)
C24H18N2O7          Mw:446.4

Preparation of the reagent
Density of the provided sample is 5mmol/L in DMSO. Dilute 500 times with neutral buffer before use.

Diaminofluorescein-2 diacetate (DAF-2 DA)

Reference
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price
Code no. Product size Price Protocol MSDS
SK1002-01 Diaminofluorescein-2 diacetate (DAF-2 DA) 1mg $522 Download
673KB
Download
175KB