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Diaminofluorescein-2 (DAF-2)

Features

  • Real time observation of NO generated by tissues or cells is available.
  • Low autofluorescence from the living sample because Diaminofluorescein-2 (DAF-2) is excited by the visible light.
  • Low damage to the cells because Diaminofluorescein-2 (DAF-2) is excited by the visible light.
  • Needless to change pH for the measurement because Diaminofluorescein-2 (DAF-2) captures NO and makes fluorescence under neutral pH condition.
  • High sensitivity and selectivity.

Principle of the measurement

Amino groups of Diaminofluorescein-2 (DAF-2) capture NO, and the DAF-2 makes fluorescence of green light with wave length of 515 nm when it’s excited by the light with wavelength of 495 nm.

Contents

DAF-2 1mg (in 0.55 mL DMSO) C20H14N2O5              Mw:362.3

Preparation of the reagent
Density of the provided sample is 5mmol/L in DMSO. Dilute 500 times with neutral buffer before use.

Diaminofluorescein-2 (DAF-2)  

Reference
Giuseppe Pezzotti, Elia Marin, Tetsuya Adachi, Alfredo Rondinella,
Francesco Boschetto, Wenliang Zhu, Nobuhiko Sugano, Ryan M. Bock,
Bryan McEntire & Sonny B. Bal (2017)
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Kuo RC, Baxter GT, Thompson SH, Stricker SA, Patton C, Bonaventura J, Epel D. Nature. 2000 Aug 10;406(6796):633-6.
Nakatsubo N, Kojima H, Kikuchi K, Nagoshi H, Hirata Y, Maeda D, Imai Y, Irimura T, Nagano T. FEBS Letters,427,263-266, 1998.
Kojima, H., Nakatsubo, N., Kikuchi, K., Kawahara, S., Kirino, Y.,Nagoshi,H., Hirata, Y., and Nagano, T. Anal. Chem. 70 2446-2453, 1998.
price
Code no. Product size Price Protocol MSDS
SK1001-01 Diaminofluorescein-2(DAF-2) 1mg $521 Download
669KB
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280KB