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[Imaging of zinc ion]ZnAF-2 / [Imaging of zinc ion with cell permeability]ZnAF-2 DA

ZnAF-2, that has structure of fluorescein connected with TPEN analog, has high specificity to Zn2+.ZnAF-2 / ZnAF-2 DA


ZnAF-2 has high solubility, so that it is not permeable through the cell membrane. ZnAF-2 DA has an additional acetyl group which make the reagent go inside the cell much easier, and the acetyl group is removed by the intracellular esterase which make the reagent stay inside the cell for long time.

ZnAF-2 / ZnAF-2 DA


  • High affinity to Zn2+ that enables us to detect low concentration of Zn2+ (dissociation constant: 2.7 nM).
  • High specificity to Zn2+ in the buffer.
  • Low background fluorescence contributes high sensitivity of Zn2+ in living sample.
  • ZnAF-2 DA is permeable through the cell membrane, so that by using the reagent, bioimaging in living cell or tissue is available.
  • ZnAF-2 DA is deacetylated by esterase in the cell, and it stays inside the cell for long time.

Principle of the measurement

Complex of ZnAF-2 and Zn2+ has green fluorescence (wavelength of 515 nm) by the excitation at 492 nm.


ZnAF-2           1mg (DMSO 0.28mL)
C34H32Cl4N4O5      Mw:718.45
ZnAF-2 DA     1mg   (DMSO 0.28mL)
C38H32N4O7           Mw:656.23

Preparation of the reagent
Density of the provided sample is 5mmol/L in DMSO. Dilute 1000 times with neutral buffer before use.

ZnAF-2 / ZnAF-2 DA

Fluorescent intensity of ZnAF-2 with various metal ion.



ZnAF-2 / ZnAF-2 DA

Fluorescence intensity of ZnAF-2 with Zn2+.



ZnAF-2 / ZnAF-2 DA


Miyoshi Y, Tanabe S, Suzuki T.
Cellular zinc is required for intestinal epithelial barrier maintenance via the regulation of claudin-3 and occludin expression. 
Am J Physiol Gastrointest Liver Physiol. 2016 311(1):G105-G116.
doi: 10.1152/ajpgi.00405.2015
McRae R, Bagchi P, Sumalekshmy S, Fahrni CJ.
Chem Rev. 2009 Oct;109(10):4780-827. doi: 10.1021/cr900223a.
Paoletti P1, Vergnano AM, Barbour B, Casado M.
Neuroscience. 2009 Jan 12;158(1):126-36. doi: 10.1016/j.neuroscience.2008.01.061. Epub 2008 Feb 15.
Que EL, Domaille DW, Chang CJ.
Chem Rev. 2008 May;108(5):1517-49. doi: 10.1021/cr078203u. Epub 2008 Apr 22.
Domaille DW, Que EL, Chang CJ.
Nat Chem Biol. 2008 Mar;4(3):168-75. doi: 10.1038/nchembio.69.
Komatsu K, Urano Y, Kojima H, Nagano T.
J Am Chem Soc. 2007 Nov 7;129(44):13447-54. Epub 2007 Oct 10.
Komatsu K, Kikuchi K, Kojima H, Urano Y, Nagano T.
J Am Chem Soc. 2005 Jul 27;127(29):10197-204.
Kikuchi K, Komatsu K, Nagano T.
Curr Opin Chem Biol. 2004 Apr;8(2):182-91.
Ueno, S.; Tsukamoto, M.; Hirano, T.; Kikuchi, K.; Yamada,MK.; Nishiyama, N.; Nagano, T. Matsuki, N.; Ikegaya, Y.J. Cell. Biol. 2002, 158, 215-220
Hirano, T.; Kikuchi, K.; Urano, Y.; Nagano, T. J. Am.Chem. Soc. 2002, 124, 6555-6562
Hirano, T.; Kikuchi, K.; Urano, Y.; Higuchi, T.; Nagano, T.J. Am. Chem. Soc. 2000, 122, 12399-12400
Code no. Product Size Price Protocol MSDS
SK2001-01 ZnAF-2 1mg $565 Download
SK2002-02 ZnAF-2DA 1mg $565 Download