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[Specific detection of Fe2+] FeRhoNox™-1

For specific detection of Fe2+

  • Specific detection of Fe2+
  • Applicable to live cell imaging of labile Fe2+

FeRhoNox™-1 is a fluorescent imaging probes for detection of iron (II). This probe is localized in Goldi, and react selectively with Fe2+. It can detect Fe2+ in Goldi of living cells.


  • For detection of Fe2+ in aqueous media
  • For detection of labile iron in living cells

The feature of FeRhoNox™-1 (after reduction)

λabs 540 nm

λFl 575 nm

Figure 1. The reaction mechanism of Fe<STRONG>RhoNox-1</STRONG>™ and Fe2+ Figure 1. The reaction mechanism of FeRhoNox™-1 and Fe2+

Figure 2. The fluorescent spectra of Fe<STRONG>RhoNox-1</STRONG>™ after treated with Fe2+ Figure 2. The fluorescent spectra of FeRhoNox™-1 after treated with Fe2+

FeRhoNox™-1 was treated with Fe2+ for 1 h at 37℃. After reacted, the fluorescence intensity increased at 575 nm.

Figure 3. Iron (II) detection of by Fe<STRONG>RhoNox-1</STRONG>™ in HepG2 cells Figure 3. Iron (II) detection of by FeRhoNox™-1 in HepG2 cells

HepG2 cells were treated with Fe(NH4)2(SO4)2 (FAS; 100 µM) for 0.5 hr, then FeRhoNox-1 (5 µM) was added and incubated for 1 hrF.

Fumiya Ito , Takahiro Nishiyama, Lei Shi , Masahiko Mori, Tasuku
Hirayama ,Hideko Nagasawa, Hiroyuki Yasui, Shinya Toyokuni ,(2016)
Biochem Biophys Res Commun. 476: 600-606
Yama K, Sato K, Murao Y, Tatsunami R, Tampo Y.
Epalrestat Upregulates Heme Oxygenase-1, Superoxide Dismutase, and Catalase in Cells of the Nervous System.
Biol Pharm Bull. 2016 39(9):1523-1530.
doi: 10.1248/bpb.b16-00332
Cancer Sci. 2015 Dec 17. doi: 10.1111/cas.12865.
Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis.
Wang Y, Okazaki Y, Shi L, Kohda H, Tanaka M, Taki K, Nishioka T, Hirayama T, Nagasawa H, Yamashita Y, Toyokuni S.
Hirayama, T.; Okuda, K.; Nagasawa, H. Chem. Sci. 2013, 4, 1250-1256.
Application Mukaide, T.; Hattori, Y.; Misawa, N.; Funahashi, S.; Jiang, L.; Hirayama, T.; Nagasawa, H.; Toyokuni, S. Free Radic. Res. 2014, 48, 990-995.
FeRhoNox™-1 is defined as RhoNox-1 in the paper.
Tomoyo Imamura, Tasuku Hirayama, Kazuhiro Tsuruma, Masamitsu Shimazawa, Hideko Nagasawa, and Hideaki Hara, Volume 129, December 2014, Pages24-30, Experimental Eye Research “Hydroxyl radicals cause fluctuation in intracellular ferrous ion levels upon light exposure during photoreceptor cell death”
Code No. Product Size Price Protocol MSDS
GC 901 FeRhoNox™-1 50μg×10 $ 498.00
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