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[Specific detection of Fe2+] FeRhoNox™-1

– For specific detection of Fe2+

  • Specific detection of Fe2+
  • Applicable to live cell imaging of labile Fe2+

FeRhoNox™-1 is a fluorescent imaging probe for the detection of iron (II). This probe is localized in Golgi, and reacts selectively with Fe2+. It can detect Fe2+ in Golgi of living cells.

Application

  • For the detection of Fe2+ in aqueous media
  • For the detection of labile iron in living cells

Redox reaction of FeRhoNox™-1 to generate fluorescence

λabs 540 nm(excitation)

λFl 575 nm(emission)

Figure 1. The reaction mechanism of Fe<STRONG>RhoNox-1</STRONG>™ and Fe2+ Figure 1. The reaction mechanism of FeRhoNox™-1 and Fe2+

Figure 2. The fluorescent spectra of Fe<STRONG>RhoNox-1</STRONG>™ after treated with Fe2+
Figure 2. Response of FeRhoNox™-1 to iron(Ⅱ)ion.

Reacting FeRhoNox™-1 with Fe2+ for 1 h at 37℃, peak of the fluorescence
appears around 575 nm.

Figure 3. Iron (II) detection of by Fe<STRONG>RhoNox-1</STRONG>™ in HepG2 cells
Figure 3. Iron (II) detection by FeRhoNox™-1 in HepG2 cells

HepG2 cells preincubated with/without Fe(NH4)2(SO4)2 (FAS: 100 µM) for 0.5 hr was incubated with 5µM of FeRhoNox-1 for 1 hr.

Figure 4. The selectivity of FeRhoNox™-1.
Figure 4. The selectivity of FeRhoNox™-1.

FeRhoNox™-1 can react with only Fe2+ selectively.

 

Figure 5. Comparison between FeRhoNox™-1’s and Golgi Marker’s staining images.
Figure 5. Comparison between FeRhoNox™-1’s and Golgi Marker’s staining images.

The Merge image shows that FeRhoNox™-1 selectively reacts with Fe2+ in Golgi apparatus.

 

To HepG2 cell

To HepG2 cell

① – Fe2+         : not added Fe2+ (100 μM Ferrous ammonium sulfate)
② +Fe2+              : added Fe2+
③ +Fe2+ +Bpy : added Fe2+ and Bpy(1 mM) as Fe2+ chelator

② shows increase of FeRhoNox™-1’s fluorescence intensity and ③ shows decrease indicating that FeRhoNox™-1 reacts with Fe2+ selectively.

To HEK293 cell

To HEK293 cell

① – Fe2+         : not added Fe2+ (100 μM Ferrous ammonium sulfate)
② +Fe2+              : added Fe2+
③ +Fe2+ +Bpy : added Fe2+ and Bpy(1 mM) as Fe2+ chelator

② shows increase of FeRhoNox™-1’s fluorescence intensity and ③ shows decrease indicating that FeRhoNox™-1 reacts with Fe2+ selectively.

Detection of internal Fe2+

Detection of internal Fe2+

Since the fluorescence intensity on ② appears decreased compared to ① by adding “Bpy”, FeRhoNox™-1 detects internal iron(II).

Bpy: cell permeable Fe2+ selective chelator (2,2-bipyridyl).

Reference
Fumiya Ito, Takahiro Nishiyama, Lei Shi, Masahiko Mori, Tasuku Hirayama, 
Hideko Nagasawa, Hiroyuki Yasui, Shinya Toyokuni, (2016)
Biochem Biophys Res Commun. 476: 600-606
Yama K, Sato K, Murao Y, Tatsunami R, Tampo Y.
Epalrestat Upregulates Heme Oxygenase-1, Superoxide Dismutase, and Catalase in Cells of the Nervous System.
Biol Pharm Bull. 2016 39(9):1523-1530.doi: 10.1248/bpb.b16-00332

Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis.
Wang Y, Okazaki Y, Shi L, Kohda H, Tanaka M, Taki K, Nishioka T, Hirayama T,  Nagasawa H, Yamashita Y, Toyokuni S.
Cancer Sci. 2015 Dec 17. doi: 10.1111/cas.12865.

Hirayama, T.; Okuda, K.; Nagasawa, H. 
Chem. Sci. 2013, 4, 1250-1256.

Application
Mukaide, T.; Hattori, Y.; Misawa, N.; Funahashi, S.; Jiang, L.; Hirayama, T.; Nagasawa, H.; Toyokuni, S. 
Free Radic. Res. 2014, 48, 990-995.

FeRhoNox™-1 is described as RhoNox-1 in the paper.

Tomoyo Imamura, Tasuku Hirayama, Kazuhiro Tsuruma, Masamitsu Shimazawa, 
Hideko Nagasawa, and Hideaki Hara, 
Experimental Eye Research 2014,129,24-30. 

Code No. Product Size Price Protocol MSDS
GC 901 FeRhoNox™-1 50μg×10 $ 498.00
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    418KB
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    152KB

Contact Information

For further information or questions on our products, please contact us, E-mail: info_itnl@goryochemical.com