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[Imaging of galctosidase] TokyoGreen®βGal

TokyoGreen®βGal is permeable through the cell membrane and is fluorescent substrate (9-(4‘-methoxy-2’-methylphenyl)- 6-(β-D-galactopylanosyloxy) -xanthen-3-one) for detecting β-galactosidase. Cell lysis or fixation is not need since TokyoGreen®βGal is taken into the cell. TokyoGreen®βGal is applicable to the experiments of gene targeting and the cell cloning by using lacZ as gene marker.

Features

  • TokyoGreen®βGal is recently-developed fluorescent chemical detecting the activity of β-galactosidase in living cells.
  • TokyoGreen®βGal is permeable through the cell membrane. Cell lysis, which is necessary for the previous colorreaction using ONPG, is not needed.
  • Applicable to the measurement using fluorescence fluoroscopy or plate reader, since fluorescence in the reaction buffer ranges uniform.
  • Able to continue culturing cells after the measurement of the β-galactosidase activation, because the reagent is removable by replacing the culture medium for several times.

Principle of the measurement

Non-fluorescent TokyoGreen®βGal is taken into the cell, is hydrolyzed by the β-galactosidase, and generates bright fluorescent TokyoGreen®. TokyoGreen® is also permeable through the cell membrane, so that generated TokyoGreen® diffuses uniformly in the culture medium and that the whole medium makes green fluorescence (510 nm) when it is irradiated by the 490 nm excitation light.

Contents

TokyoGreen®βGal 1mg (5 mM in DMSO 0.4mL)
C27H26O9               Mw:494.49

TokyoGreen®-<i>β</i>Gal

 

Please refer to application note

Reference
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Tamura A, Ikeda G, Seo JH, Tsuchiya K, Yajima H, Sasaki Y, Akiyoshi K, Yui N.
Sci Rep. 2013;3:2252. doi: 10.1038/srep02252.
Matsuura T, Hosoda K, Ichihashi N, Kazuta Y, Yomo T.
J Biol Chem. 2011 Jun 24;286(25):22028-34. doi: 10.1074/jbc.M111.240168. Epub 2011 Apr 29.
Ayame H, Morimoto N, Akiyoshi K.
Bioconjug Chem. 2008 Apr;19(4):882-90. doi: 10.1021/bc700422s. Epub 2008 Mar 13.
Y. Urano, M. Kamiya, K. Kanda, T. Ueno, K. Hirose, T. Nagano : J. Am. Chem. Soc. 2005, 127, 4888-4894
price
Code no. Product size Price Protocol MSDS
 SK4001-01  TokyoGreen®-β Gal 1mg $598 Download
422KB
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183KB