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[Visualizaion of βgalactosidase in the cell and detection of SA-b-Gal, senescent cell.]GlycoYELLOW™-βGal

~For gene analysis of lacZ in ‘live’ cell~

  • Usable for Live Cell Imaging
  • High sensitivity
  • High retention ability in cell

GlycoYELLOW™βGal is a specific fluorescent probe for detection of β-galactosidase. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ.

Because GlycoYELLOW™βGal is almost non-fluorescent in the absence of β-galactosidase, this probe exhibits good S/N ratio.

GlycoYELLOW™βGal is suitable for…

      • Gene analysis by fluorescent imaging.
      • Monitoring transfection efficiency and live cell imaging of transfected cell.
      • Study in promoter or enhancer.

Feature of GlycoYELLOW™βGal

λabs 525 nm*

λfl 543 nm

*Excitation by 488nm laser is also possible.

Figure 1. Mechanism of fluorescent generation of GlycoYELLOWTM-<i>β</i>Gal; fluorescent dye (HMDER) was generated in hydrolysis reaction by β-galactosidase.

Figure 1. Mechanism of fluorescent generation of GlycoYELLOW™βGal.

Usable for Live Cell Imaging

Usable for Live Cell Imaging

Mean fluorescence intensity in cells of
GlycoYELLOW™βGal

5.6times increase

293/LacZ 735.3

HEK293 130.2

Figure 2. Live cell imaging of 293/LacZ (b-galactosidase expressing cell line) and HEK293 cell by using GlycoYELLOW™βGal. Comparing these cell lines, strong fluorescence derived from GlycoYELLOW™βGal was observed inside 293/LacZ cell.

High Sensitivity

Figure 2. Fluorescent spectra of GlycoYELLOW(TM)-<i>β</i>Gal before and after hydrolysis by β-galactosidase; GlycoYELLOW(TM)-<i>β</i>Gal was treated with β-galactosidase (3 unit) for 30 min at 37 ℃. Fluorescent intensity at 547nm was increased 37 times after reaction.

Figure 3. Fluorescent spectra of GlycoYELLOW™βGal before and after hydrolysis by β-galactosidase; GlycoYELLOW™βGal was treated with β-galactosidase (3 unit) for 30 min at 37℃. Fluorescent intensity at 547nm was increased 37 times after reaction.

Good retention ability inside the cell
(a)

Good retention ability inside the cell
(b)

Good retention ability inside the cell

Figure 4. (a) Time-lapse observation of 293/LacZ cell (β-galactosidase expressing cell) treated by GlycoYELLOW™βGal. (b) Time course of the fluorescent intensity averages in area 1-5 of figure 3a. Error bars : ±s.d.
This result indicated that GlycoYELLOW™βGal possesses excellent retention ability inside the cell.

Please refer to application.

Reference
Mako Kamiya, Daisuke Asanuma, Erina Kuranaga, Asuka Takeishi, Masayo Sakabe, Masayuki Miura, Tetsuo Nagano, and Yasuteru Urano J. Am. Chem. Soc. 2011, 133, 12960-12963
GlycoYELLOW™βGal means HMDER-βGal in the communication.
Price
Code No. Product Size Price Protocol MSDS
GC601 GlycoYELLOW™βGal 50μg×10 $598.00
  • Donwload
    488KB
  • Donwload
    273KB

GlycoYELLOW™βGal were commercialized by Goryo Chemical, Inc. under the guidance of Prof. Yasuteru Urano. (Laboratory of Chemical Biology and Molecular Imaging, Graduate School of Medicine, The University of Tokyo)

Contact Information

Further information or questions on our products, please contact us, E-mail: info_itnl@goryochemical.com