Application Note / Goryo Chemical, Inc.

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POLARIC™ for labeling nucleic acid and protein

- Available for labeling bio samples

POLARIC™-isothiocyanate

Example of POLARIC™-isothiocyanate

Examples of POLARIC™-isothiocyanate structure

  • Useful for labeling protein and oligonucleotide, etc.
  • The only thing you have to do is mix substrate and fluorescent material.
  • Reactions proceed without using any condensation reagent.
  • Purification by dialysis or chromatography are available due to POLARIC™’s high water solubility.

SDS-PAGE

Samples of collagen labeled by POLARIC™-isothiocyanate were run in SDS-PAGE and analyzed UV light.

POLARIC™-COOH, NH2

This dye has COOH group or NH2 group.
Available for labeling to amino terminus, carboxyl terminus or amino acid side chain.

Examples of POLARIC™-COOH structure

Examples of POLARIC™-COOH structure

  • Available for labeling peptide and proteins, etc.
  • Available for labeling peptide by solid-phase synthesis.

SDS-PAGE

Samples of amyloid-β labeled by POLARIC™-COOH by solid-phase synthesis were run in Native-PAGE and analyzed UV light.

POLARIC™-NHS

POLARIC™ has N-Hydroxy succinimidyl ester which activate COOH group.

Examples of POLARIC™-NHS structure

Examples of POLARIC™-NHS structure

  • Available for labeling proteins and oligonucleotide, etc. Espetially water soluble proteins.
  • Undergo the reaction only have to mix.
    Not necessary condensing agent.
  • Available for purifying with dialysis and chromatography due to its high hydrophilicity.

SDS-PAGE

Samples of amyloid-β labeled by POLARIC™-COOH by solid-phase synthesis were run in Native-PAGE and analyzed UV light.

  • Also, We offer variety of reactive forms, POLARIC™ structure as you like.

Further information or questions on our products, please contact us, E-mail: solvato@polaris-t.com