TokyoGreen®–βGal

N/A

$598

Clear
- +
Add to WishlistAdd to Compare
Code No. Product Size Price
SK4001-01 TokyoGreen®-β Gal 1mg $ 598.00

TokyoGreen®–βGal is permeable through the cell membrane and is fluorescent substrate (9-(4‘-methoxy-2’-methylphenyl)- 6-(β-D-galactopylanosyloxy) -xanthen-3-one) for detecting β-galactosidase. Cell lysis or fixation is not need since TokyoGreen®–βGal is taken into the cell. TokyoGreen®–βGal is applicable to the experiments of gene targeting and the cell cloning by using lacZ as gene marker.

Features

  • TokyoGreen®–βGal is recently-developed fluorescent chemical detecting the activity of β-galactosidase in living cells.
  • TokyoGreen®–βGal is permeable through the cell membrane. Cell lysis, which is necessary for the previous colorreaction using ONPG, is not needed.
  • Applicable to the measurement using fluorescence fluoroscopy or plate reader, since fluorescence in the reaction buffer ranges uniform.
  • Able to continue culturing cells after the measurement of the β-galactosidase activation, because the reagent is removable by replacing the culture medium for several times.

Principle of the measurement

Non-fluorescent TokyoGreen®βGal is taken into the cell, is hydrolyzed by the β-galactosidase, and generates bright fluorescent TokyoGreen®. TokyoGreen® is also permeable through the cell membrane, so that generated TokyoGreen® diffuses uniformly in the culture medium and that the whole medium makes green fluorescence (510 nm) when it is irradiated by the 490 nm excitation light.

Contents

TokyoGreen®βGal 1mg (5 mM in DMSO 0.4mL)
C27H26O9               Mw:494.49

Reference

R. Kawasaki, Y. Sasaki, K. Katagiri, S.Mukai, S. Sawada, K. Akiyoshi (2016)
Angew.Chem., Int. Ed. 55: 11377–11381

Inuzuka S, Nishimura K, Kakizawa H, Fujita Y, Furuta H, Matsumura S,
Ikawa Y (2016)
J Biochem 160: 153-162.

Okano T, Matsuura T, Suzuki H, Yomo T.
ACS Synth Biol. 2014 Jun 20;3(6):347-52. doi: 10.1021/sb400087e. Epub 2013 Sep 4.

Tamura A, Ikeda G, Seo JH, Tsuchiya K, Yajima H, Sasaki Y, Akiyoshi K, Yui N.
Sci Rep. 2013;3:2252. doi: 10.1038/srep02252.

Matsuura T, Hosoda K, Ichihashi N, Kazuta Y, Yomo T.
J Biol Chem. 2011 Jun 24;286(25):22028-34. doi: 10.1074/jbc.M111.240168. Epub 2011 Apr 29.

Ayame H, Morimoto N, Akiyoshi K.
Bioconjug Chem. 2008 Apr;19(4):882-90. doi: 10.1021/bc700422s. Epub 2008 Mar 13.

Y. Urano, M. Kamiya, K. Kanda, T. Ueno, K. Hirose, T. Nagano :
J. Am. Chem. Soc. 2005, 127, 4888-4894

S.Inuzuka, H.Kakizawa, K. Nishimura, T. Naito, K. Miyazaki, H.Furuta S. Matsumura, Y.Ikawa (2018)
Genes Cells.In press. DOI: 10.1111/gtc.12586

 

SKU: N/A Category:

Start typing and press Enter to search

Shopping Cart

No products in the cart.