|SK4001-01||TokyoGreen®-β Gal||1mg||$ 598.00|
TokyoGreen®–βGal is permeable through the cell membrane and is fluorescent substrate （9-(4‘-methoxy-2’-methylphenyl)- 6-(β-D-galactopylanosyloxy) -xanthen-3-one） for detecting β-galactosidase. Cell lysis or fixation is not need since TokyoGreen®–βGal is taken into the cell. TokyoGreen®–βGal is applicable to the experiments of gene targeting and the cell cloning by using lacZ as gene marker.
- TokyoGreen®–βGal is recently-developed fluorescent chemical detecting the activity of β-galactosidase in living cells.
- TokyoGreen®–βGal is permeable through the cell membrane. Cell lysis, which is necessary for the previous colorreaction using ONPG, is not needed.
- Applicable to the measurement using fluorescence fluoroscopy or plate reader, since fluorescence in the reaction buffer ranges uniform.
- Able to continue culturing cells after the measurement of the β-galactosidase activation, because the reagent is removable by replacing the culture medium for several times.
Principle of the measurement
Non-fluorescent TokyoGreen®–βGal is taken into the cell, is hydrolyzed by the β-galactosidase, and generates bright fluorescent TokyoGreen®. TokyoGreen® is also permeable through the cell membrane, so that generated TokyoGreen® diffuses uniformly in the culture medium and that the whole medium makes green fluorescence (510 nm) when it is irradiated by the 490 nm excitation light.
TokyoGreen®–βGal 1mg (5 mM in DMSO 0.4mL)
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