ProteoGREEN™-gGlu

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GC 801ProteoGREEN™ -gGlu20μg×10$ 650.00

– For specific detection of cancer cells –

  • Cancer cells specific
  • High S/N ratio
  • GGT (γ-glutamyltranspeptidase)-activatable fluorescent probe

ProteoGREEN™-gGlu is cancer-selective fluorescent imaging probe. This probe is rapidly activated by g-glutamyltranspeptidase (GGT), which is known to be tumor-associated enzyme, to give highly fluorescent compound.
The membrane-permeable ProteoGREEN™-gGlu is applicable to live cell or in vivo imaging of cancer cells.

ProteoGREEN™-gGlu has almost non-fluorescent in the absence of GGT. Treatment of ProteoGREEN™-gGlu with GGT results in a fluorescence turn-on with high S/N ratio.

in vivo imaging

Live cell imaging

Application

  • For fluorescence imaging of cancer cells

Previous data shows fluorescence detected from cell line below.

Cell line from human ovarian cancer
SHIN-3, SK-OV-3, OVCAR-3, OVCAR-4, OVCAR-5, OVCAR-8, A2780, A2780 PTX22, IGR-OV1, Hey-A8, CaOV3

Cell line from human lung cancer
A549

Cell line from human biliary cancer
HuCCT1

Cell line from human hepatic cancer
HepG2

Cell line from human uterocervical cancer
HeLa

Others
Colon cancer artificially generated in a mouse human breast cancer tissue
*On the contarary, we have heard almost no increased fluorescence of ProteoGREEN in HUVEC. (not cancer cell).

The feature of ProteoGREEN™-gGlu

λabs 496 nm

λFl 525 nm

Figure 1. The reaction mechanism of ProteoGREEN™-gGlu and GGT

Figure 2. The fluorescent spectra of ProteoGREEN™-gGlu after treated with GGT

ProteoGREEN™-gGlu was treated with GGT for 30 min at 37℃. After reacted, the fluorescence intensity markedly increased at 525 nm (S/N ratio).

Figure 3. Live cell imaging of cancer cells or HUVEC cells

Each cells were treated with ProteoGREEN™-gGlu (2 µM) for 1 h. The reaction was completely inhibited by treatment with GGT selective inhibitor.

Reference

K. Kawakami, Y. Fujita, Y. Matsuda, T. Arai, K. Horie, K. Kameyama, T. Kato, K. Masunaga, Y. Kasuya, M. Tanaka, K. Mizutani, T. Deguchi, M. Ito (2017)
BMC Cancer 17: 316 DOI: 10.1186/s12885-017-3301-x

Ali Behrooz, Peter Waterman, Kristine O. Vasquez, Jeff Meganck, Jeffrey D. Peterson, Ilias Faqir, and Joshua Kempner (2017)
Optics Letters 42:2964-2967, DOI: 10.1364/OL.42.002964

Yoichi Miyata, Takeaki Ishizawa, Mako Kamiya, Suguru Yamashita, Kiyoshi Hasegawa, Aya Ushiku, Junji Shibahara,
Masashi Fukayama, Yasuteru Urano & Norihiro Kokudo  (2017)
Scientific Reports 7:3542  DOI:10.1038/s41598-017-03760-3

Yuko Nakamura, Toshiko Harada, Tadanobu Nagaya, Kazuhide Sato, Shuhei Okuyama, Peter L. Choyke, Hisataka Kobayashi (2016)
Oncotarget. 7(32):51124-51137.

Fluorescent imaging of superficial head and neck squamous cell carcinoma using a γ-glutamyltranspeptidase-activated targeting agent: a pilot study Takeshi Mizushima, Shunsuke Ohnishi, Yuichi Shimizu, Yutaka Hatanaka, Kanako C. Hatanaka, Hidetaka Hosono, Yoshimasa Kubota, Mitsuteru Natsuizaka, Mako Kamiya, Shouko Ono, Akihiro Homma, Mototsugu Kato, Naoya Sakamoto, and Yasuteru Urano
BMC Cancer, 2016, 16, 411.  

Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green.
Ueo H, Shinden Y, Tobo T, Gamachi A, Udo M, Komatsu H, Nambara S, Saito T, Ueda M, Hirata H, Sakimura S, Takano Y, Uchi R, Kurashige J, Akiyoshi S, Iguchi T, Eguchi H, Sugimachi K, Kubota Y, Kai Y, Shibuta K, Kijima Y, Yoshinaka H, Natsugoe S, Mori M, Maehara Y, Sakabe M, Kamiya M, Kakareka JW, Pohida TJ, Choyke PL, Kobayashi H, Ueo H, Urano Y, Mimori K.
Sci Rep., 2015, 5, 12080

Rational Design of Highly Sensitive Fluorescence Probes for Protease and Glycosidase Based on Precisely Controlled Spirocyclization Sakabe, M.; Asanuma, D.; Kamiya, M.; Iwatate, RJ.; Hanaoka, K.; Terai, T.; Nagano, T.;
Urano, Y.
J. Am. Chem. Soc., 2013 , 135, 409-414. 

Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe.
Mitsunaga, M.; Kosaka, N.; Choyke, PL.; Young, MR.; Dextras, CR.; Saud, SM.; Colburn ,NH.; Sakabe, M.; Nagano, T.; Asanuma, D.; Urano, Y.; Kobayashi, H.
Gut, 2013, 62, 1179-1186.

Novel live imaging techniques of cellular functions and in vivo tumors based on precise design of small molecule-based ‘activatable’ fluorescence probes. Urano Y.
Curr Opin Chem Biol.,2012, 16, 602-608.

Rapid cancer detection by topically spraying a γ-glutamyltranspeptidase-activated fluorescent probe.
Urano, Y.; Sakabe, M.; Kosaka, N.; Ogawa, M.; Mitsunaga, M.; Asanuma, D.; Kamiya, M.; Young, M. R.; Nagano, T.; Choyke, P. L.; Kobayashi, H.
Sci. Transl. Med., 2011 , 129, 1-10.
ProteoGREEN™-gGlu is defined as gGlu-HMRG in the paper.

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