ICG Near Infrared Fluorescent Dyes
~For in vivo imaging using near infrared fluorescent dyes~

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Code No.ProductSizePriceApplication & Note
A501-01ICG-NHS ester1 mg × 1ask usFor in vivo fluorescent imaging. Labeling of antibodies by amide bonding.
Exmax / Emmax (nm): 790/830
A501-051 mg × 5ask us
A501-101 mg × 10ask us
A502-01ICG maleimide1 mg × 1ask usLabeling thiols of proteins with maleimide.
Exmax / Emmax (nm): 790/830
A502-051 mg × 5ask us
A502-101 mg × 10ask us
IM110 ICG CBT 1mg$ 498.00Labeling selectively cysteine residues in proteins or antibodies by cyanobenzothiazlole groups.
IM1115mg$ 1,880.00
IM11210mgask us
IM113ICG azide1mg$ 498.00Labeling of antibodies or
other proteins through azide-alkyne
cycloaddition (click reaction.)
IM1145mg$ 1,880.00
IM11510mgask us
A501-21 ICG-NHS ester labeling kit 5 timesask usICG-NHS ester labeling kit provide a convenient means for labeling fluorescent probe to a small amount of antibody or protein (20–100 µg), and supply everything needed for labeling reactions. Exmax / Emmax (nm): 790/830
IM119 IR-820 NHS ester 1mg$ 498.00For in vivo fluorescent imaging. Labeling antibody by amide bond. Excitation/Emission maximum (nm):710/820
IM1205mg$ 1,130.00
IM12110mgask us
IM125 IR-820 CBT 1mg$ 498.00Labeling selectively cysteine residues in proteins or antibodies by cyanobenzothiazlole groups.
IM1265mg$ 1,410.00
IM12710mgask us

 

  • For Labeling with NHS esters, maleimides or through click reaction.
  • Labeling kits are available.
  • 【Contract labeling service】 Products are synthesized in our lab in Japan.

NHS series (Most commonly used method for labeling)

  • Labeling of antibodies by amide bonding.
  • Labeling kits are available

Maleimide series(Labeling the thiol groups of cysteine residues)

  • Labeling the thiol groups of cysteine residues.
  • All you have to do for the labeling is just mixing.

CBT series

(Labeling of cysteine residues.)

Azide series

(Labeling easily through click reaction.)

Flow Chart of contract ICG-antibody labeling service

Procedure for ordering

Ask us to inquire about price and scheduling.

Filling out the application documents and sending them by FAX

Please download all application documents and fill out them. Send us the filled-out documents by FAX:+81-11-351-1822, before send us your sample.

Sending your sample

Send us your sample and application documents to the above address. Be sure to pack the sample carefully to avoid drying, leakage or corruption of the vial. The sample should reach us by a weekday morning.

Deliverables

Work report Final products of contracted work, such as purified antibodies.

Indocyanine Green Labeling Kit

Indocyanine Green-NHS (succinimidyl ester) is near-infrared fluorescent probe that binds to amino acid residues without any condensing agents. Not only proteins or antibodies but also oligo-nucleotide with amino acid at the end can be labeled. This kit contains reagents and tools that required for the labeling for five times with Indocyanine Green-NHS and purification. One package of Indocyanine Green-NHS is optimum for the labeling of 300 μg of proteins, such as IgG.

Indocyanine Green Labeling Kit
Contents of the kit
  • Indocyanine Green-NHS× 5
  • Dimethylsulfoxide500μL × 1
  • Reaction Buffer5mL × 1
  • Washing Buffer10mL × 1
  • Spin column for ultrafiltration× 5

Labeling of antimicrobial peptide with NHS (Example)

Reference

Boudewijn E. Schaafsma, MD, J.Sven D. Mieog, MD, Merlijn Hutteman, MSc, Joost R. van der Vorst, MD, Peter J.K. Kuppen, PhD, Clemens W.G.M. Löwik, PhD, John V. Frangioni, MD, PhD, Cornelis J.H. van de Velde, MD, PhD, and Alexander L. Vahrmeijer, MD, PhD,* J Surg Oncol. 2011 Sep1; 104(3): 323-332., “The clinical use of indocyanine green as a near-infrared fluorescent contrast agent for image-guided oncologic surgery”

K. Sigmundsson, J. R.M. Ojala, M. K. Öhman, A. M. Österholm, A. Moreno-Moral, A. Domogatskaya, L. Y. Chong, Y. Sun, X. Chai, J. A. M. Steele, B. George, M. Patarroyo, A. S. Nilsson, S. Rodin, S. Ghosh, M. M. Stevens, E. Petretto, K. Tryggvason. (2018)
Matrix Biol.pii: S0945-053X(18)30052-0 DOI: 10.1016/j.matbio.2018.03.018

D. Matsuoka, H. Watanabe, Y. Shimizu, H. Kimura, Y. Yagi,R. Kawai, M. Ono, H. Saji (2018)
Bioorg. Med. Chem. In press DOI: 10.1016/j.bmc.2018.03.015

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