HMSiR labeled Goat anti-mouse IgG (whole)
- Superresolution imaging without UV laser irradiation is possible. Additions of thiols and oxygen scavengers are not required.
- Spontaneously blinking fluorophore for single molecule localization microscopy (SMLM) suitable for STORM/PALM microscopes.
HMSiR™-labeled secondary antibodies can be used for staining fixed cells.
HMSiR™ is a fluorescent probe which shows spontaneous blinking in physiological conditions. It enables superresolution imaging without high-power laser irradiations, without additions of thiols or oxygen scavengers, which were necessary for the previous dSTORM observation.
Figure 1. Superresolution images of microtubules in HeLa cells.
Fixed and permeabilized HeLa cells were incubated with mouse anti-α-tubulin IgG (1/300 diluted of 625901, BioLegend) at room temperature for 2 hours. After washing the antibody, cells were incubated with 10 μg/mL of HMSiR™-labeled Goat anti-mouse IgG (H&L) (A202-01) at room temperature for 2 hours. The images were obtained using NIKON N-STORM microscope.
Figure 2. Comparison of SMLM superresolution images to conventional fluorescent images.
Fixed and permeabilized HeLa cells were incubated with rat anti-tubulin IgG (1/300 diluted YL1/2, NB600-506, Novus Biologicals) for 2 hours. After washing the antibody, cells were incubated with 10 μg/mL of HMSiR™-labeled Goat anti-rat IgG (H&L) (A203-01) at room temperature for 2 hours. Cells were observed using NIKON N-STORM microscope. (left) Averaged image of 5000 images of HMSiR, representing conventional fluorescent images. (right) Result of SMLM image analysis from 5000 images. Analyses were performed using NIS-Elements (NIKON).
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